Methods for animal preparation, training, recording, and data analysis were similar to previous studies (Fukushima et al. 2000; Akao et al. 2005; Kasahara et al. 2006), except for head-free pursuit and are summarized here briefly.
Our methods for animal preparation, training, recording, and data analysis are described elsewhere in detail (e.g., Fukushima et al. 2000; Shichinohe et al. 2009) and are briefly summarized here.
Methods for animal preparation, training, recording, and data analysis were basically similar to those in previous studies (Fukushima et al. 2000; Akao et al. 2005; Kasahara et al. 2006; Fukushima, Kasahara, Akao, Kurkin, et al., 2009), except for trunk rotation, and are summarized here briefly.
Currently, machines are used in sample preparation, recording of results and electronic transfer of data to various agencies.
We only collected limited dietary data (food preparation records) which hindered the understanding of the impact on dietary behaviors.
The relaxant effects of the tested substances were taken as the percent change in spontaneous contractions of the preparation recorded immediately before the addition of the substances.
Measures of process evaluation included monitoring of children's physical activity (activity time and intensity) and food preparation records, and fidelity of intervention protocol implementation.
The observed relaxant effects of test substance were quantified as the percent decrease in spontaneous contractions of the preparation recorded immediately prior to the addition of test substances.
In some experiments, the same slice preparation, recording and staining procedures were carried out for the pyramidal neurons in layers 3 and 5 of GRS, layer 2 of the barrel field cortex (BF) of adult rats for comparison.
Based on the analysis of food preparation records, the children in the intervention center were served meals with reduced fat (−9.76 g, p > .05), increased fiber (+2.69 g, p < .04) and fruits (+43 g, p > .0001), compared to the children in the control center.
