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cDNA libraries were prepared according to Illumina sequencing sample preparation protocols.
Prevalent sequencing library preparation protocols for FFPE samples usually require more than 5 ng starting DNA.
Future ethnopharmacological studies on the medicinal plants examined here should incorporate traditional preparation protocols.
Most chromosomal preparation protocols for fish have been designed for a single species.
Therefore, improved data preparation protocols for SEM research are urgently needed.
Besides the aforementioned elements, this study attempted to modify other important factors of chromosomal preparation protocols.
The endoscopists who performed the procedures were blinded to the preparation protocols.
TTAS is free of ligation step, independent of specific template DNA sequence, and compatible with prevalent sequencing library preparation protocols.
Potassium chloride (KCl 0.075 M) is one of the most commonly used hypotonic solutions in chromosomal preparation protocols (Table 1).
Traditional preparation protocols may also call for more dilute solutions compared to the methanol extractions used in this study.
The use of an internal control (18S rRNA) allowed comparison of sample preparation protocols and amplification efficiencies between samples.
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