All the preparation procedure, from capillary pretreatment until the MSP is ready to use in CEC, were made in less than four hours in mild conditions.
A DNA sample preparation procedure, such as buffer replacement and DNA concentration adjustment, is not essential for MspJI digestion, which permits a simple DNA library preparation procedure from amplicons.
To avoid possible sources of variation in the data, individual laboratories developed standardized protocols involving all the various steps of the sample preparation procedure, starting from tumor sample collection, through sample processing, total RNA isolation, cDNA synthesis, cRNA synthesis and labeling, target fragmentation, microarray hybridization, to washing and staining protocols.
The preparation procedures for obtaining carbon from hair are very simple, namely, reductant or deionized water activation process followed by hair carbonization under argon atmosphere at 800 °C for 2 h.
The authors need to either provide citations to existing evidence that their sample preparation procedure removes nucleotide from RF3, or demonstrate that it is removed.
To decrease the cost of analysis, the whole sample preparation procedure was miniaturized (from 5 g to 0.5 g of sample), but all the conditions used (i.e., temperature, time of hydrolysis, ratios of sample weight/acid volume/extraction solvent volume/neutralization agent volume) remained the same.
According to the characteristics of oil sludge, the preparation procedure was designed learning from conventional asphaltic-type shale stabilizer preparation method.
This initial injury could be observed in all slices and is attributable to minimal cell death originating from the preparation procedure and to influential effects regarding the handling and maintenance of the slice cultures over the 14-day cultivation time period.
A simple preparation procedure to produce conductive graphene coatings from graphenide solution under inert atmosphere is achieved.
All fractions recovered from the membrane preparation procedure were analysed for protein content by immunobloting (as above) after dilution to a normalised volume (corresponding to 270 mL/fraction).
We prepared for cortical bone specimens from bovine femurs following the previous preparation procedure [ 16].
