For ChIP-seq sample preparation, pooled ChIP reactions (10 ng) were used to prepare a single library using the ChIP-seq Sample Prep Kit (Illumina) according to the manufacturer's protocol.
During the sample preparation, pools were created from equal volumes of aliquots of all samples.
During the sample preparation, pools were created from equal volumes of subaliquots of all samples in the study.
[The exact fraction numbers pooled varied by preparation; pools were normalized batch-to-batch by collecting each species peak as identified by immunoblot intensity].
Transfected TRH cells were purified by fluorescence activated cell sorting, various cell preparations pooled, and their transcriptome compared to that of GFP- hypothalamic cells.
We subjected the m/z peak intensity list collected to a two-way ANOVA statistical analysis with Bonferroni post-test correction to extract m/z peaks significantly over-represented in the two distinct resting monocyte preparations (pooled data from 7 volunteers).
In vitro transcription and preparation of endoribonuclease-prepared pools of complex siRNA (esiRNA) was carried out as described previously [ 50].
Prior to library preparation RNA pool quality was assessed on the Agilent bioanalyzer (www.agilent.com).
At the time of preparation, a pool was created from equal amounts of small aliquots of each study sample, which was treated identically to a sample.
The two lipid extracts from each cell preparation were pooled, subjected to mild alkaline hydrolysis.
JPT, KIC and YLF co-ordinated the provision of PRBC, and contributed to the supernatant preparation and pooling.
