Total protein preparation, SDS-PAGE, immunoprecipitation and immunoblotting analysis and enhanced chemiluminescence were carried out as described [3], [4].
Total cell lysate protein preparation, SDS-PAGE, and immuno-blotting: as described [80], [82], except Cell Signaling antibodies, where supplier's protocol was followed precisely.
Cell or tissue lysate preparation, SDS-PAGE, transfer of SDS gels to an Immobilon-P membranes (Millipore) were performed as previously described [36].
Cell extract preparation, SDS-PAGE and immunoblotting procedures have been described previously [ 12].
Cell lysate preparation, SDS-PAGE and Western blot, antibody incubation and signal detection were carried out as described previously [ 30].
For ArAT I preparation, SDS-PAGE analysis showed the presence of five visible protein bands (Fig. 2a).
Cell lysate preparation, SDS-PAGE, and immunoblotting, antibody incubation and signal detection were performed as described previously [ 67].
Total cell lysate (TCL) preparation, SDS-PAGE, immunoprecipitation (IP), and immunoblot (IB) analysis methods have previously been described [ 8, 9].
To check the purity of the tPA preparations, SDS-PAGE was performed using a 5% stacking gel and a 10% separating gel in Tris-glycine running buffer.
In this section, a typical InCHlib use consisting of data preparation and web page deployment is described.
