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Preparation of tissue explants -- 12.4.2. Isolation of chondrocytes -- 12.4.3.
Microsurgical preparation of tissue explants avoids the destruction of the organ-specific tissue architecture.
Extracellular matrix (ECM) has drawn a broad attention for preparation of tissue engineering scaffolds and stem cell study.
Preparation of tissue engineered bone constructs to repair large size defects is limited by the difficult supply of oxygen and nutrients to cells deep inside the constructs.
The ability to topographically mimic the surface features of collagen fibrils is an important step in the preparation of tissue engineering scaffolds.
First to use the microtome (a mechanical device for slicing thin tissue sections), glacial acetic acid, potassium bichromate, and Canada balsam in the preparation of tissue samples for microscopic examination, Purkinje also described the experimental effects on humans of camphor, opium, belladonna, and turpentine (1829) and the visual images produced by poisoning with digitalis and belladonna.
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The preparation of the tissue and purification by Sep-Pak C18 cartridge (Millipore Corporation, Milford, MA, USA) was performed as described in Berge et al (2004a).
The preparation of matrix-matched tissue standards has been described by Hare et al. [ 90] in detail.
Recovered co-cultured cell sheets can be manipulated, moved and sandwiched with other structures, providing new useful constructs both for basic cell biology research and preparation of tissue-mimicking multi-layer materials through overlaying co-cultured cell sheets.
Fixation and preparation of tissues for immunohistochemistry was performed as described in ref. 71.
Sequential processing steps involved in the diagnostic preparation of tissue--gross dissection, selection of tissue blocks, processing, imbedding in paraffin, cutting and mounting on glass slides, all funnel through the final stages of staining, and cover-slipping in preparation for review and diagnosis by a pathologist.
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