Preparation of protein samples, labeling, application to the Panorama® Cell Signaling Array chip and data analysis was performed according to manufacturers' instructions using the proprietary solutions and equipment provided (Panorama® Cell Signaling Antibody Microarray, Sigma, St . Louis MO.
The first authors to write on the preparation of niello and its application to silver were Eraclius and Theophilus, in or about the 12th century, and Benvenuto Cellini, during the 16th.
Among these steps are: (1) masking, (2) cleaning, (3) surface preparation, (4) preparation of the coating, (5) application of the coating, (6) drying and curing, (7) mask removal, (8) inspection, and (9) rework.
Researchers may be increasing their preparation time regardless of the application process because the competition is getting stronger.
At the first project meeting, a protocol for the submission of the contractor's requisition should be established so the contractor has clear direction regarding the preparation of the initial application for payment.
LN contributed to the conception and design of the study including preparation of the funding application.
All authors were involved in design of the protocol and preparation of the Human Research Ethics Committee application.
Authors' contribution: All authors were involved in design of the protocol and preparation of the Human Research Ethics Committee application.
