Sentence examples for preparation of template from inspiring English sources

a Preparation of template by PnP process.

This work provides improvements in four steps of long distance RT-PCR (L-RT-PCR): (i) preparation of a viral stock, (ii) preparation of template RNA, (iii) reverse transcription and (iv) amplification of the cDNA by LD-PCR.

Culture of human EJ30 bladder carcinoma cells, cell synchronisation in late G1 phase by mimosine, and preparation of template nuclei were performed as described [32], [42].

PCR amplification for the preparation of template DNA for pyrosequencing was carried out by AmpliTaq Gold DNA Polymerase LD Applied Biosystemss) [21].

Laboratories employ different in-house methods for preparation of template DNA as well as different amplification systems.

However, the method suffers from a disadvantage of tedious procedures for the preparation of template-free nanoparticles for further application.

After preparation of templates, the PIC assembly reaction was performed as described previously (Yudkovsky et al, 2000).

Preparation of bait template and IVV template libraries, and the procedure of IVV screening were described in detail in our previous report [21].

Care was taken to avoid contamination by doing all procedures like isolation of DNA, PCR master mixture preparation, addition of template, PCR, and gel electrophoresis in separate rooms.

The whole process of the LAMP assay from the preparation of DNA template to endpoint detection only required 80 min even whereas the conventional PCR required 4 hours starting from DNA template to the visualization of the amplicons using agarose gel electrophoresis.

A DC power supplier (MP6003, Megatek, Germany) was used to apply electric field during two-step anodization process in preparation of AAO template.