Sentence examples for preparation of synaptic from inspiring English sources

First, we used two different subcellular fractionation protocols; one using an iodixanol gradient and another one focusing on the preparation of synaptic fractions, and measured the γ-secretase activity in the fractions obtained.

DOI: http://dx.doi.org/10.7554/eLife.07794.011 10.7554/eLiFigure94.012 figure 2—figure supplement 1. Preparation of synaptic proteins enriched Triton-Resistant-Membranes (TRM) from mouse brain.

We also found high γ-secretase activity in crude preparations of synaptic vesicles and synaptic membranes and these results were confirmed by the enrichment of the γ-secretase components in a highly pure synaptic vesicle preparation.

Inclusion of postsynaptic proteins was based on the presence of proteins in proteomics analyses of the synapse, including preparations of synaptic membrane fractions as well as the postsynaptic density.

An alternative explanation, however, is that APP is processed to Aβ in synaptic vesicles, a proposal which is supported by complementary data from Frkyman and colleagues who recently found Aβ using biochemical methods in a somewhat cruder preparation of isolated synaptic vesicles [13].

In this preparation, the organization of synaptic inputs is preserved and the dendritic plane of PNs is perpendicular to the slice with a variable descending angle (Figure 9A).

Synaptic transmission relies on two distinct molecular pathways for neurotransmitter release: the preparation of neurotransmitter-filled synaptic vesicles to a docked state at the active-zone and action potential-driven opening of voltage-gated calcium channels (VGCCs) causing rapid elevation of intracellular calcium in the vicinity of these vesicles.

However, in each of these studies, non-physiological or intense sustained stimulation was used in dissected preparations to trigger cycling of synaptic vesicles.

To overcome the shortcomings of in vitro preparations and to identify modulations of synaptic input, chemical modification of the bath solution has been used to increase background synaptic activity in brain slices [21] [23].

Therefore, the integration of synaptic input in slice preparations may not truly reflect the conditions found in vivo [6] [9].

On the other hand, time-dependent changes in basal [Ca2+]i may represent only one factor that interferes with the expression of synaptic plasticity in slice preparations.