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Preparation of stage V-VI oocytes from Xenopus laevis and synthesis of capped off run-off poly(A+) cRNA transcripts from linearized cDNA templates (pCMV vector) were performed as previously described [13].
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RCA and Y-LY generated cleared and stained bone preparations of staged specimens, and performed in situ hybridization experiments.
Both data sets were generated from the same mRNA preparation of L1 stage animals and as such are a direct comparison of these two gene expression-measuring techniques.
This is of course most important in preparation for stage three of Economic and Monetary Union.
Based on the results shown in Figures 3 and 4, sex ratios were estimated in preparations of late stage IV and stage V (non-activated) gametocytes.
CK was responsible for the preparation of the stage-specific trypanosome populations.
Although preparation of early-stage bone-marrow-derived mesenchymal stem cells (BM-MSCs) is critical for successful cell transplantation therapy, no culture system offers a sufficient number of early-stage BM-MSCs for cell transplantation.
The procedures for preparation and injection of stage IV Xenopus laevis oocytes were as previously described [19].
That is the reason why the main goal of the preparation stage of the project was to establish working relationships with the representatives of the city and state administration.
With the exception of the preparation stage of change, these variables were also predictors that individuals would say that they would react by smoking less cigarettes.
The unexpectedly high rates of motivation (preparation stage of the TTM model) for a change at baseline may be an indicator of a possible bias.
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CEO of Professional Science Editing for Scientists @ prosciediting.com