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§ 173.462 Preparation of specimens for testing.
D.J.V. thanks E. Metz, E. Przybyszewski, and A. Takekawa for help in the field and B. Harmon and J. Jette for preparation of specimens.
In this study, two types of concrete mixes were used for preparation of specimens.
But the truth is that we know less about some of these very bulky animals than those of more moderate dimensions, as their very size renders the collection and preparation of specimens of them more difficult.
An efficient study design includes provisions for processing of the original samples, such as cryopreservation, DNA isolation, and preparation of specimens for exposure assessment.
In the preparation of specimens, the governing factors including deposition conditions, annealing temperature, and annealing time, were arranged following the orthogonal table of five-level and six-factorial (L25 56)) design.
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The preparation procedure of specimens consisted in a preliminary tooth crown and a root pulp tissue removal.
Dependent on number of PCR cycles, modalities of PCR and various preparations of specimen, different assays of various sensitivity and specificity were subsequently developed for detection of circulating EBV DNA [ 1, 7- 10].
The specimens were then prepared for SBS testing following the same protocol described for the preparation of enamel specimens, except for the acid-etching step which was carried out using 10% hydrofluoric acid for 90 s (Condac Porcelana, FGM).
When considering convenience in the preparation of the specimen, the rectangular specimen is particularly recommended, although the effective span length is restricted.
More preparation of both specimens will be necessary to say definitively whether that's the case.
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