Sentence examples for preparation of sequencing from inspiring English sources

Preparation of sequencing templates was carried out following standard protocols, employing FIREPol polymerase (Solis BioDyne).

The size distribution of selectively bound DNA is concentration independent (Figure 2), which supports the preparation of sequencing libraries from dilute samples.

The preparation of sequencing templates was carried out following standard protocols, employing FIREPol polymerase (Solis BioDyne).

The amount of RNA harvested from a single embryo is not sufficient for preparation of sequencing libraries using standard methods and necessitates amplification following cDNA synthesis.

That said, he suspects the effect might be due to sequencing artifacts, as the preparation of sequencing libraries can introduce biases.

All RNA extractions yielded sufficient total concentrations of RNA (1 10 µg/µl) for preparation of sequencing libraries, with RNA integrity numbers higher than 8.

We did not use paired-end (PE) libraries because we wanted to keep the preparation cost of sequencing as low as possible.

The first process was the preparation of sequences, during which A. thaliana DNA was placed into a single linear sequence and then screened for unique five base sequences.

Because of the use of FLEA-PCR amplicons for DNA sequencing, preparation of Illumina sequencing libraries is associated with the formation of adapter dimers (ILLUMINA, 2015).

JMD, PFM, GFB and AHN conceived of the study, designed and coordinated the study, participated in preparation of viral DNA, sequencing studies, sequence analyses, and helped to draft the manuscript.

Cross-contamination after library preparation (e.g. during preparation of the sequencing run) can be easily identified and filtered from the final sequencing data when sample-specific barcodes are included in the libraries and determined during sequencing [ 9, 10].