Sentence examples for preparation of probe from inspiring English sources

The preparation of probe, pre-hybridization, hybridization and immunological detection were all performed according to the protocol of DIG-High Prime DNA Labeling and Detection starter Kit (Roche, USA).

Total RNA for preparation of probes for microarray hybridization was prepared from material from each treatment: T. virens alone (Tv, 5 experiments), in interaction with maize (M + Tv, 3 experiments) and with tomato (T + Tv, 3 experiments).

Cartridges can be plumbed between reaction vessels for intermediate purification or for final preparation of probes that do not require high-performance liquid chromatography (HPLC) purification.

The introduction of different functionalities at the periphery of the coordinating moiety of β-diketones and the physico-chemical properties arising from the related complexes are considered also in correlation with their potential in the preparation of probes and devices, where molecular components are essential.

The clone collection is available for preparation of probes for transcriptome analyses.

The preparation of probes and hybridization to MG430 2.0 chips were performed following Affymetrix guidelines.

The microarray-based MIP preparation and capture experiment followed MIP standard-operating procedures with modifications in the preparation of probes (manuscript in preparation) [ 17].

Using PCR we obtained representative genomic Rumex sequences for each family as described in Materials and Methods section (see Preparation of Probes for Fluorescence In Situ Hybridization).

Generally, the ISH protocol used in this study consisted of five steps, which were (a) the preparation of probes, (b) preparation of tissue sections, (c) pretreatment of tissue sections to increase the accessibility of probes to γ-chain mRNA, (d) hybridization of probes to the tissue and posthybridization washes, and (e) detection and visualization of the nucleic acid hybrids.

We note that previous studies using zinc chelators in vivo in mammals have demonstrated acceptable safety profiles.[ 32] We therefore believe that Gd ⋅1 provides a first-stage model for the preparation of probes, which might ultimately be of clinical utility.

TR contributed to the gene list, probe and overall microarray design, and performed cloning and preparations of probes for microarray manufacturing.