Sentence examples for preparation of primary from inspiring English sources

Preparation of primary cultures of neonatal cardiomyocytes was achieved by trypsin/collagenase digestion of ventricles harvested from 3- to 5-day-old neonatal Sprague Dawley rats following decapitation.

The synthetic pathways explored toward these dimers revealed unexpected features, such as the direct formation of secondary and tertiary quaterthiophene amines through a procedure initially developed for the preparation of primary amine exclusively.

The preparation of primary human hepatocytes is a time consuming, logistically demanding, and expensive process.

We thank Margie Maronski and Kevin Miyashiro for preparation of primary neurons and Frances Vito for sectioning of neuropathological tissue.

Age-matched groups of 8 to 12-week-old mice were used for preparation of primary hepatocytes.

The procedures for preparation of primary mouse astrocyte cultures were adapted from a previously described protocol [29].

All measurements have been performed in duplicates and in three independent experiments with different preparations of primary cell cultures.

The panel of human "immortalized" ovarian (HIO) epithelial cell lines [17], two preparations of primary human ovarian surface epithelial cells [36], and ovarian cancer cell lines were analyzed.

Total RNA was isolated from 8 preparations of primary HOSE cells (from 7 persons, here, C.47 and D.47 were split from the same HOSE preparations but were cultured by different lab staff), 6 HIO lines which are HOSE cells transfected with SV40-T-antigent to prolong culturing life span, and 6 lines of ovarian cancer cells (Figure 5).

The downstream product of nuclear phospho-ERK activity, monitored as expression of the immediate early gene c-Fos, was weak in the primary breast and ovarian cells despite the elevated phospho-ERK levels (Figure 1A,B), and nearly undetectable in some preparations of primary cells at early passage numbers.

Different preparations of primary human MC served as controls and were cultured as previously described [ 14].