Preparation of precision-cut lung slices (PCLS) was performed as previously described.
Three samples were used to standardize and optimize the preparation of precision-cut breast tumor slices, and, from these, explants of a defined size and thickness (4 mm in diameter and 250 300 μm thick) were obtained for ex vivo culture under controlled conditions.
Thus, AIP lesions significantly reduced the PMv-M1 facilitation of 1DI seen during preparation of a precision grip (RM-ANOVA cTBS × interval × grasp interaction: F = 5.96, p = 0.032; Figure 2A), as well as the PMv-M1 facilitation of ADM during preparation of a whole-hand grasp (RM-ANOVA cTBS × interval × grasp interaction: F = 7.36, p = 0.027; Figure 2A).
The level of preparation and precision at the FA's National Football Centre is impressive.
Given the low event rates in the included trials, establishing the cardiovascular safety of a preparation with sufficient precision would require a trial with more than 100 000 patients followed up for at least one year.
Yet the preparation of RNA affects the precision of microarray measures and therefore the ability to accurately quantify the content of an RNA sample [11].
With the use of robotic sample preparation to optimize precision, this assay should be adaptable to clinical environments.
This paper describes the design of the Repository to perform a semi-automated subsampling procedure, offering high degree of flexibility and precision in the preparation of NM vials for customers, while guaranteeing the safety of the operators, and environmental protection.
Previous assays in biological fluids have often suffered from complex, multistage sample preparation, poor precision and substantial limitations in the number of these complex analytes that could be assessed at low levels found in biological fluids [ 15, 22].
Indeed, our measurements confirm that there is negligible variation in H2B/H3 between donors, demonstrating that our sample preparation results in precision of measurements.
The Relative Standard Deviation (%RSD = SD/χ of the replicate values X 100%; χ is mean value) was calculated to give an indication of sample preparation and analytical precision.
