The first and most important step in any study – no matter the methods and the techniques used - is the preparation of the study methodology, as this constitutes the "roadmap" from start to end [ 55].
JTL: Description of methodology, preparation of programming, calculation and presentation of results, contribution to introduction, discussion and conclusion.
As there was limited previous research in this area and the available studies did not report key details of methodology, careful preparation was required for an adequately powered investigation.
The discrepancies in the literature were ascribed in a large extent to a lack of rigorous methodology, preparation of samples as well as anti-P-gp antibodies varying from a study to another [ 32, 33].
Combining the protocols of Gatenby et al. [ 17], and Rasmussen, [ 18] for SBB staining, we achieved optimal lipofuscin visualization in cell cultures and tissues with the following methodology: Preparation of SBB solution: 0.7gr of SBB BDHH, Vizas, Athens, Greece) was dissolved in 70% ethanol, covered with parafilm and thoroughly stirred overnight at room temperature.
KS optimized the methodology for preparation of 2-DE gels and prepared the gels.
HOA contributed in the development of methodology and manuscript preparation.
SC participated in the conception of the study, development of methodology and manuscript preparation.
DK participated in sample preparation, mass spectrometry, data analysis, verification of methodology, and drafted the manuscript.
The cytoprotective effect of PRMs was evident irrespective of the preparation methodology.
The complete methodology for preparation of SLNs was shown in Fig. 1.
