Your English writing platform
Free sign upSuggestions(5)
Exact(2)
We describe here the construction of Gateway-compatible vectors, pBGP1-DEST and pPICZα-DEST, for rapid and convenient preparation of expression plasmids for production of secretory proteins in Pichia pastoris.
Preparation of expression vectors for gene cloning in cells of the genus Lactobacillus is challenging, mainly due to the unusually high level of genetic diversity.
Similar(58)
CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations.
For preparation of the expression construct, the whole gene fragment encoding N-terminal scFv and C-terminal apoptin (GeneBank accession number: KY884983) was digested with NcoI/HindIII from pGEM scFv apoptin plasmid and cloned into the NcoI/HindIII site of pET-28a.
Consequently, by this method, preparation of shRNA expression plasmids for high-throughput screening is facilitated.
JW and YLZ carried out the plant material preparation, PV infection, RNA extraction, preparation of digital expression libraries, sequence analysis, and contributed to data interpretation and manuscript writing.
While no longer providing the promoter/TSS specificity of nanoCAGE, LNA-mediated template-switching could become an option in the preparation of digital expression libraries or revised versions of RNA-seq protocols [ 9].
However, it is difficult to relate the monkey FRET signals to these studies because of the differences in species, preparations, and methods of expression of the sensor.
We now report the preparation of efficient recombinant expression systems for KynB from three bacterial sources and their purification.
The preparation of caspase-14 expression vector and empty vector was previously described [ 27], and the cDNA sequences of the vectors were confirmed by DNA sequencing.
The two vectors were then fused by Cre-recombination yielding a 'master plasmid', which was used to transform DH10α MultiBac cells for preparation of the MultiBac expression vector.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com