Electrophysiological recording from E18.5 ex vivo brain stem preparations showed depression of endogenous respiratory motor root output under baseline conditions and in response to the excitatory neuropeptide, substance P, confirming abnormal respiratory phenotype in Hprt- cre, Phox2b∆8 mice (Fig. 1d), in keeping with other mouse models of CCHS [ 14, 26, 44, 54].
To examine the effect of arsenite on solubility of endogenous TDP-43, preparation of soluble/insoluble protein were performed.
The low ATP concentration in the cell lysates was most likely due to the quick turnover of endogenous ATP during the preparation of the lysates.
Intravenous administration of Fungizone, an amphotericin B preparation complexed with deoxycholate, augmented the induction of endogenous TNF in response to a second stimulus with intravenous doses of FK23 (heat-killed Enterococcus faecalis).
Our attempt to measure labeling of endogenous SVOP in synaptic vesicle preparation was hampered by the low abundance of SVOP in synaptic vesicles and the lack of an efficient antibody for immunoprecipitation.
Without complexities of sample preparation and potential toxicity of exogenous biomarkers, autofluorescence imaging of endogenous fluorophores promises a noninvasive method for functional measurement of the retina [ 13].
Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated.
This possibility is consistent with the detection of endogenous retroviral RNA species in exosomal preparations [37].
Alternate matrix method by using distilled water as an alternate analyte-free matrix for the preparation of calibration standards (CS) was used to avoid the influence of endogenous Sr in serum on the quantification.
To avoid MT bundling associated with the overexpression of GFP TIP150, we selected a preparation in which cells express GFP TIP150 to a level comparable with that of endogenous TIP150 (supplementary Fig S3 online).
