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For preparation of control PRF by the conventional method, the anticoagulant was not added.
A lentiviral vector carrying a shRNA against luciferase (luc-shRNA) was used in the preparation of control virions.
Non infected Vero cells were used for the preparation of control antigen.
For preparation of control, 1.3 mL of methanol was used instead of extract solution.
Non-infected Vero cells grown and handled under similar conditions were used for the preparation of control antigen.
A crucial aspect, in particular for quantitative analysis, is elimination of artefacts during preparation of control samples for flow cytometry.
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The immunohistochemical procedure and preparation of controls were carried out according to the manufacturing company's standards and guidelines.
In order to further evaluate the intracellular levels of Hsp70 expression, we made protein preparations of control, M. smegmatis and M. avium infected macrophages at different times after infection and blotted them with anti-Hsp70 antibody (Fig. 3A).
Injectable in situ gels have caused much attention and have been studied quite a lot in the fields of drug delivery, possibly due to their ease of preparation, ease of control in quality, non-use of organic solvents, site-specific delivery, prolonged action periods, and improved patient compliance (Matsumura and Maeda 1986).
To minimize variability, sample extractions, addition of IS and preparation of quality control (QC) samples were prepared in the same day using the same batch of solvents.
The preparation of a control strategy begins with a thorough steady-state analysis.
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