Sentence examples for preparation of constructs from inspiring English sources
AK carried out the mutation analysis, preparation of constructs and transfected FDC-P1 cells, FACS analysis, and drafted the manuscript.
Bimolecular fluorescence complementation (BiFC), including preparation of constructs, was performed in N. benthamiana epidermal cells according to [ 50].
Current results however provide encouraging evidences on the use of hypergravity as a physical stimulus suitable for the preparation of constructs for regenerative medicine purposes.
The exploration of strong hypergravity values indeed opens opportunities to a better understanding of cellular responses to extreme conditions, and to the preparation of constructs with desired phenotype for cell transplantation and drug screening procedures.
By driving cell behaviour, hypergravity could represent a simple, safe, and cost-effective approach in the biomedical field that could be used in the in vitro preparation of constructs for tissue engineering and drug screening [ 45– 47] and that could also be considered for futuristic preclinical approaches in the treatment of pathological conditions [ 48].
Previously we reported a method called DelsGate for rapid preparation of deletion constructs for protoplast-mediated fungal transformation systems, which is based on Gateway® technology.
The preparation of fibroblast/composite constructs was investigated using cell culture as a first stage in mimicking the dermal/epidermal structure of skin.
This method requires preparation of cDNA constructs encoding donor-acceptor fusion fluorescent proteins differing as widely as possible in FRET efficiency.
We would like to thank N. Archidiacono for providing the alpha-satellite probes, M. Iannicelli for helping with preparation of the constructs, D. Manzoni for excelent technical assistance with cell cultures, W.C. Earnshaw, A. Choo and D. Diolaiti for critical discussions.
NC performed preparation of DNA constructs.
Expression plasmids: The preparation of plasmid constructs expressing PPARα, γ and δ has been described previously.
