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Preparation of cell cultures.
Additional file 3: Surface roughness parameters of individual endothelial, THP-1 cells and SMC and preparation of cell cultures.
This new technology exhibits utility for the preparation of cell sources required for cell transplantation as well as microglial function analysis.
Preparation of cell extracts and CO-difference spectra determination on them (600 400 nm with a sampling interval of 1 nm) were done as described by Dikshit and Webster (1988) using a Shimadzu UV-1800 spectrophotometer.
To examine how USP33 regulates Robo1 protein stability, H1299 cells were transfected with control siRNA (Ctrl) or siUSP33, and treated with cycloheximide (CHX, an inhibitor of protein synthesis) for different periods of time before the preparation of cell lysates.
Preparation of cell extracts and Western blotting analysis were performed as described [15].
Preparation of cell lysates, PAGE and immunoblotting was carried out as described previously [43].
Preparation of cell extracts and cell fractionation were performed as described previously [14] with some modifications.
Preparation of cell lysates and western blotting was performed as previously described [80].
For phosphorylation assays, WEHI 274.1 cells were stimulated with BMPs for 30 minutes before preparation of cell lysates.
At various times after inoculation, whole lungs were harvested for extraction of total RNA or preparation of cell suspensions.
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