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Additional file 1: Preparation of buffer systems.
The standard prescribed procedure was employed for the preparation of buffer solutions (Vogel 1961).
Double-distilled water was used for preparation of buffer and standard solutions.
All salts for the preparation of buffer solutions were obtained from Helicon (Moscow, Russia).
From the same sample, 6 ml aliquots were presented to volunteers in sets of three, where only one sample was the test and other two were deionised water used in preparation of buffer.
Absolute ethanol and HPLC-grade methanol (Sigma, St . Louis MO, USA) were used for extraction procedures and preparation of buffer for HPLC analysis.
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Ultrapure Milli-Q water (Millipore, Belford, MA, USA) was used for the preparation of buffers and solutions.
Preparation of buffers was carried out at the appropriate temperatures by mixing the appropriate dibasic and basic salts.
Materials: All chemicals for the preparation of buffers, as well as the heparin and the lacmoid were purchased from Sigma Aldrich.
Since the kit comes with transposase pre-loaded with adapters and a proprietary buffer, reducing all reaction components in the same proportions as the input DNA also removes the need for custom preparation of buffers.
In addition, a sharp drop in publications describing mitochondrial functions, respiration in particular, during the last decade was probably associated with the ubiquitous usage of Milli-Q water for preparation of buffers.
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