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Preparation of brain membrane: Crude synaptosomal membranes from rat brain were prepared according to the method described by Lohse [21].
The preparation of brain slices has been previously described [10], [30].
In order to minimize variation introduced by RNA amplification, a relatively large amount of tissue was used for the preparation of brain lysates (∼1000 brains per immunoprecipitation experiment).
Preparation of brain homogenates.
After 3 days the animals were killed for preparation of brain slices.
Spleen sample preparation was similar to the preparation of brain samples using a methanol soak.
Similar(45)
Moreover, to confirm absence of PrPSc in the preparation of brain-derived atypical PrPres amplified in dgPMCAb, the products of the 18th dgPMCAb round were subjected to six serial PMCAb rounds (Fig. 2d).
Both types of pericapillary deposit of Aβ can be demonstrated in paraffin sections, in smear preparations of brain [ 28] or on vessels isolated by digesting away the surrounding brain tissue [ 4].
The NO-consuming activity of brain tissue was assessed by comparing the NO concentration profile in control buffer and various preparations of brain tissue on application of the NO donor DETA/NO.
Regarding the 5-HT1B receptor, an analogous context-dependent bidirectional modulation has been reported in in vitro preparations of brain slices containing the optic tract and the LGN (Seeburg et al. 2004) or the ventral posteriomedial nucleus of thalamus and the somatosensory cortex (Laurent et al. 2002).
Preparation of whole brain leukocytes from unperfused brain tissue was adapted with modifications from a method previously described [61].
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