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RNA-Sequencing (RNA-Seq) experiments have been optimized for library preparation, mapping, and gene expression estimation.
In summary, we describe in detail the preparation, mapping, and data analysis approaches for reproducible cardiac tissue slice-based investigations into AP and CaT dynamics.
It should be noted that that equivalency only holds for the specific approach (i.e. RNA source, library preparation, mapping parameters) used in [ 29].
Taken together, these results indicate that the library preparation, mapping and SNP detection parameters utilized in the present study identified a high quality set of SNPs for downstream use.
However, their results critically depend on the quality of the reference genome and mapping software, and they are not specifically designed to handle errors [ 13], which come from various sources including unwanted RNA fragments during the library preparation, mapping errors (due to sequencing errors and/or repeats), or "dark matters" from inter-genetic and intron regions [ 14].
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Proper experiment design (sample preparation, map definition) and data treatments (viewing formats, profile creation) are essential for success, especially if the sample contains features that are smaller than the diffraction limit (10 μm at 1000 cm−1).
He explained, in great detail, civil preparation maps and impacts from past hurricanes.
The preparation map, however, also includes activity in motor structures (vPM, dPM, SMA, and PPC [posterior parietal cortex]) involved in motor preparation, leading to a conservative estimate of brain areas involved in motor execution in the remaining Hexe.
Results were based on observations obtained in 10 Scn5a+/ΔKpreparationsons and 12 WT preparations mapped over 15 20 s/preparation.
In preparation for mapping this mutation, RSS 26 1 (M4) was crossed to ecotype Landsberg erecta (L er).
The aim of this study was to refine experimental conditions for successful tissue slice preparation, optical mapping, and data analysis.
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