M. bovis BCG fraction II which supports in-vitro replication of DNA from M.tb oriC (manuscript under preparation) was utilized.
Additionally, although a single metal-enhanced preparation was utilized in this work for compatibility with both MALDI and SIMS, future work will include applying traditional MALDI matrixes in a microdroplet array in order to extend the mass range to include detection of proteins and other macromolecules of interest.
These enzyme preparations were utilized in the hydrolysis of insoluble wheat straw and corn fiber xylan rich fractions.
Preparations were utilized to examine the contractile response to phenylephrine (1×10−8 3×10−4 M added cumulatively) and the vasodilatory actions of acetylcholine (10−8 10−4 M), carbachol (10−9 10−4 M), ATP (3×10−5 M) and the NO donor SIN-1 (10−4 M), each added in the presence of phenylephrine.
In some experiments a 10,000 MW cut off of PE preparations was utilized to test for the presence of shorter polymers.
To generate the various complementary and comparative glial species the following additional growth factor preparations were utilized: recombinant EGF and PDGF-AA (R&D Systems).
To generate the comparative neuronal subtype species the following additional growth factor preparations were utilized: recombinant mouse β-NGF (R&D Systems), human BDNF (BioVision), recombinant BMP2 (gift from Genetics Institute) and recombinant LIF (Chemicon).
To generate the various neural stem, progenitor and more differentiated glial species the following growth factor preparations were utilized: recombinant bFGF (Collaborative Biomedical Products), recombinant EGF, N-Shh and PDGF-AA (R&D Systems) and BMP2 (gift from Genetics Institute).
To generate the various neural stem, progenitor and more differentiated neuronal subtype species the following growth factor preparations were utilized: recombinant bFGF (Collaborative Biomedical Products), N-Shh and recombinant mouse β-NGF (R&D Systems), human BDNF (BioVision), BMP2 (gift from Genetics Institute) and LIF (Chemicon).
