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When the Tox preparation is applied as a second stimulus, a strong [Ca2+]cyt elevation without refractory feature is observed in WT roots, irrespective of whether CWE, EPM or EPS were the first stimuli.
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Briefly, 5 μL from a fungal secretome preparation was applied on the gels prepared with glycol-chitin (0.3 mg/mL) or chitosan DA 35% (0.1 mg/mL).
The wound was stapled, and an antibacterial preparation was applied to the incision area.
Different approaches for the layer preparation were applied.
The following steps of data preparation are applied equally to the different datasets extracted.
The bottle was labelled and an expiry date of 2 months from the date of preparation was applied.
To discern postsynaptic changes, glutamate, the putative excitatory neurotransmitter for this preparation was applied directly to the bathing medium in order to bypass the presynaptic release process.
For this purpose, planar and cross-section preparation were applied including mechanical grinding, polishing and argon ion-beam etching followed by deposition of a thin carbon film on both sides.
The crude immune IgY preparation was applied to the human serum Affi-Gel 15 pre-clearing column and allowed to run through.
Briefly, 8 10 µL of protein preparation were applied to glow-discharged, carbon-coated Formvar grids (Electron Microscopy Sciences, Washington, PA) for 10 20 min. The solution was gently wicked off using Whatman grade-1 qualitative filter paper.
After dialysis of the corresponding fraction against 200 volumes of buffer A, the Mpl preparation was applied onto 0.25 ml of cobalt resin (TALON, Clontech) for a further purification-concentration step to eliminate a 30-kDa contaminating protein frequently co-purified with His-tagged proteins, which likely is the SlyD protein [52].
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