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Densitometric analysis demonstrated that His6-VanYn preferentially accumulated (90%, lanes 4 and 8 of Figure 2B) in the cell wall fractions obtained by the step of spheroplast preparation, independently on the localization of the His6-tag (at the C-terminus or at the N-terminus of the protein).
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First, it consistently yields highly pure PrP preparations, independently of the amount of insoluble and protease-resistant PrP in the starting material.
The search was limited to trials performed with testosterone, including testosterone esters and dihydrotestosterone preparations, independently of delivery.
Overall the IP/VP ratios were higher for the low volume preparations independently of the purification method used and ca. 5 - 20% of the particles purified from low volumes were biologically active, whereas only 1 - 9% purified from larger volumes were infectious.
Target cDNA samples were prepared independently for both untreated and partially depleted aliquots and equal amounts of each target preparation were hybridized to replicate microarrays.
The entire sample preparation was independently repeated three times.
The test replicates were taken from the same tissue DNA preparation and independently digested, size-selected, labeled, and hybridized to the array with similarly processed, but differentially labeled, spleen DNA.
Slide preparations were independently reacted with monoclonal anti-Hsp60 and polyclonal anti-CytC (BD Biosciences, San Jose, CA, USA) or anti-AIF (Chemicon, Billerica, MA, USA).
This allows nurses and midwives who have completed a prescribing preparation programme to independently prescribe a wide-range of medications.
The exam preparation course was independently led by two peer tutors.
Test performance, interpretation of test results, data analysis, and manuscript preparation were conducted independently by the authors.
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