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During the preparation, in the presence of NH4+ Beta zeolite (Si/Al = 12.5), CoCl2 precursor decomposes under helium stream without evaporation, leading to the stabilization of bare Co2+ at the exchange cationic sites as revealed by spectroscopic tools.
Finally, the application of the ROL-Lew-pH 7 preparation in the presence of 20% dioxane and 5 °C allowed us to obtain the (R -isomer of the monoesteR -isomer E value of 24 (ee = 92%) in a 97% yield (of monoesthes).
Bone marrow-derived mast cells (BMMC) were differentiated from bone marrow cell preparation in the presence of IL3 and stem cell factor (SCF), as described previously [23], [24].
As mentioned above, one of the major criteria is the resistance of the liposomal preparation in the presence of serum.
The presence of granules in the dense fraction was confirmed by incubation of the preparation in the presence of Acridine Orange.
EC [Ca2+]i and vascular displacement were determined in PE pre-contracted preparation in the presence of cumulative supplement of ACh (a receptor-dependent agonist) or ionomycin (a receptor-independent agonist).
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This sample is tagged with a C-terminal Myc and isolated from HEK293 cells pre-cultured for 24 h with sodium butyrate, a histone deacetylase inhibitor, prior to extract preparation and prepared in the presence of sodium vanadate, a phosphatase inhibitor (Dr L. Mull, OriGene Technologies, personal communication).
The anchorage-independent cell growth on soft agar assay showed that by 8 to 14 days the number of cell colonies was reduced by 50% relative to control preparations in the presence of progesterone receptor isoform A plus progestin (P <.0001, both Hec50 and KLE cell lines) and by 90% in the presence of progesterone receptor isoform B plus progestin (P <.0001, both Hec50 and KLE cell lines).
Time-course experiment revealed the adverse effect of the preincubation of either antigenic preparations in the presence of iDC on the presentation to CTL, as detectable by proliferation.
A variety of pNP substrates (Sigma-Aldrich, St . Louis MO) were used to determine the loss of enzymatic activity of commercial enzyme preparations in the presence of lignin.
While suppression correlated well to restoration of phage viability, the opposite was true when we examined fluorescence of the various phage preparations in the presence of D:: eGFP expression.
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