All the steps of tissue preparation, glycerol gradient centrifugation and BN-PAGE were performed at 4 °C.
Each group was cultured at 37°C for 24 h; in the end of culture, medium was centrifuged (3000 r/min) for 5 min; the precipitation was collected, washed and fixed for specimens preparation, after gradient dehydration, air dry and gold spray, the specimens were observed under a SEM to evaluate the morphology changes caused by S.s bacteriocin.
The behavior of a closed co-extrusion process for the preparation of gradient-index polymer optical fibers is modeled theoretically.
The preparation of gradient-purified binary-ethylenimine (BEI -inactivated FMDV C-S8c1 has BEI -inactivatedviously described {Bahnemann, 1975 #2026}.
Sucrose gradient preparation: a portion (2-3 mL) of the microvillus-enriched preparation and the basal membrane-enriched preparation was overlaid on the sucrose gradient.
This article describes the preparation of hybrid gradient porous surfaces by using the breath figures approach.
To study the potential of CASA parameters in frozen-thawed donor semen before and after preparation on silica gradient as predictors of pregnancy in IUI with donor semen cycles.
The assay of oxysterols requires completely different sample preparation, LC conditions (gradient), and mass spectrometry (ion source) from those used in this study.
The use of techniques, including density-gradient preparation, electrophoretic separation, microfluidics, high-magnification sperm morphology selection, and hyaluronic acid binding, is discussed.
For quantitative comparison of extracellular vesicle release, we preferred an ultracentrifugation protocol to sucrose gradient preparations.
