This introduction provides the basic definition and description of the methodology for the newcomer and describes the steps typically followed in its most standard canonical form (preparation, generation, structuring, representation, interpretation and utilization).
Schistosome eggs were isolated from urine samples with >100 eggs/10 ml of urine for soluble egg antigen preparation, generation of parasite stages and for immunolocalization.
24 32 In this study, the method described by Trochim was used, involving six steps: preparation, generation of statements, structuring of statements, representation of statements in the form of a concept map, interpretation and utilization.
Amplicons were combined at roughly equal amplification intensity ratios, purified using an AxyPrep DNA Gel Recovery Kit (AXYGEN) and submitted to the next-generation sequencing laboratory at Majorbio Bio-pharm Technology Co.,Ltd., Shanghai, China for Illumina paired-end library preparation, cluster generation, and 250-bp paired-end sequencing.
Sample preparation, cluster generation and sequencing were performed according to the manufacture's protocols with minor modifications (Illumina paired-end cluster generation kit GA II v1, 36-cycle sequencing kit v1.2 and v1.3).
They encompass RNA preparation, the generation of cDNA, library preparation, and the sequencing reactions themselves.
The fuel processor integrates fuel preparation, steam generation, sulfur removal, pumps, blowers and controls.
The facility scientists provide expertise for designing large-scale genotyping and sequencing projects, sample preparation, data generation, and data analysis.
Our neuroscience research investigates the neural basis of movement preparation and generation using a combination of electro-/opto-physiological, behavioral, computational and theoretical techniques.
The gelation and equilibrium swelling of the gels in water were characterized while systematically varying the polymer concentration at preparation, dendrimer generation, and mole ratio of dendrimer endgroups to PEG endgroups.
An improved blended data method was developed for preparation and generation of solar radiation gridded datasets for SILO Queensland Governmentt database containing point and gridded daily climate data for Australia from 1890 till present designed for crop and pasture modelling.
