Sentence examples for preparation frozen from inspiring English sources

The Wistar Institute, a research center in Philadelphia, made the vaccine and has kept a few drops of material used in its preparation frozen since 1957.

Prior to library preparation, frozen DNA stock was diluted to a working solution of 50 ng/μl as recommended by Illumina (CA, USA) and was quantified by agarose gel analysis.

For DNA preparation, frozen stocks were picked to 1.2 ml of YT broth + chloramphenicol in deep 96-well plates and cultured with shaking for 20 h at 37°C.

For quantification of cytochrome c in whole worm extract and in mitochondrial preparations, frozen samples with known protein concentration were mixed with Laemmli buffer, heated at 99°C for 5 min and equal amounts of protein were loaded on gels.

A 30 µl aliquot from the final preparation was frozen and forwarded for RPM-Flu analysis.

For each tissue preparation method (frozen or de-paraffinised), a separate neural net was developed.

The membrane preparation was frozen in liquid nitrogen and stored at −80 °C.

Cells to be used for BBM vesicle (BBMV) preparation were frozen immediately in liquid nitrogen and stored at −80°C until required.

Extended Experimental Procedures Sample Preparation Snap frozen HCC tissue samples were obtained from 19 patients who underwent surgery at the Centre Hépatobiliaire, Paul-Brousse Hospital (Table 1 ).

The clear supernatant fluid with its active lysin obtained during the final stage of lysin preparation was frozen at −20°C [ 6].

Then, PRP and PPP preparations were frozen and stored at −20 °C until further used (usually within 2 weeks).