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An affinity-purified GST preparation from a single digestive gland was used as the protein source with a specific activity (mean±SE) of 561±25 µmol min−1 mg protein−1.
A single total nucleic acid preparation from a single aliquot of a single specimen is simultaneously assayed for possible detection and illumination of specimen-specific gene sequences from each of the 188 independent target pathogen gene sequencing detector tiles on the array.
Dog striatum offered enough tissue to allow preparation from a single animal while mouse tissues were pooled.
Both of these samples were encased in exactly the same type of vials; we suspect that both suppliers were selling the same preparation from a single manufacturer that was made many years ago.
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Genome-sequencing efforts have primarily involved culturable organisms because high-purity DNA preparations from a single isolate can be obtained.
Third, to analyze the within-platform variations, Cy3- and Cy5-labeled cDNAs were prepared from a single preparation of total RNA from unstressed cells, mixed together and hybridized to a microarray.
If extracts from a specimen can be identified for which deep high-throughput sequencing of mtDNA shows that a single mtDNA genome is present with minimal or absent indication of any additional mtDNA, this shows that the DNA preparation derives from a single individual.
Of these systems, brain slices have the advantage of allowing investigators to obtain measurements from multiple preparations, taken from a single animal, while also maintaining the native three-dimensional architecture of the neuronal tissue.
In summary, we demonstrate the utility of measuring multiple analytes from a PRP preparation in a single step to aid the diagnosis of LOAD.
This work presents a photoinducedly electrochemical preparation of Prussian blue from a single sodium nitroprusside and insertion of cetyltrimethylammonium cations into Prussian blue as counter ions.
Each whole-mount preparation of submucosa obtained from a single biopsy was permeabilized for 3 hours in phosphate buffered saline (PBS)/NaN3 containing 1% (v/v) Triton X-100 and 10% (v/v) horse serum and then incubated with antibodies against phosphorylated alpha-synuclein (1 5000, WAKO, Osaka, Japan) and PGP9.5 (1:10,000; Ultraclone Limited, UK).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com