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The discrepancy between results of different studies can be attributed to several factors, including geographical location of the plant, parts of plants used for extracts preparation, extraction protocol, such as solvent, temperature, and time [ 10].
In the EDA studies, there was submission of the respective solvents [51,53,56,57] or of HPLC-grade water [58] through the same or part of the procedures that were applied to samples (i.e. sample preparation, extraction, fractionation).
With the rapid growth in the area of DNA diagnostics, there is an URGENT need for the development of a micro total analysis system which can do all the three main steps involved during nucleic acid-based diagnostics: sample preparation, extraction of the DNA, detection and quantification on one SINGLE chip.
To ensure blinding, information on the substrate type, spiked BCG cell loads, and each stage of matching (sample preparation, extraction, nano-spectrophotometer data for yield and quality of DNA and PCR amplification results) were scanned into the database.
Preparation, extraction, and cleanup were as described by Covaci and Voorspoels (2005).
MN had the main responsibility for this research; performed sample preparation, extraction and GC/MS metabolite analysis; wrote the manuscript.
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Essentially all steps of RNA-preparation (extraction, amplification, in-vitro transcription, labelling), hybridization, washing and signal detection can have significant effects on the extracted "apparent" expression values seen between different samples with consequences for subsequent downstream applications.
Zingiber zerumbet powder (2.0 g) was stirred in aqueous ethanol in preparation for extraction using the microwave extraction system.
PTWvA: Sample preparation, DNA extraction, and MLVA analyses of samples RQJvdP: Sample preparation, DNA extraction, qPCR, and MLVA analyses of samples DNdH: Sample preparation, DNA extraction, qPCR, and MLVA analyses of samples CBEMR: co-writer of manuscript, provided DNA of rat spleens for MLVA analyses BJvR: funding and co-writer of the manuscript.
This powder was used for feed preparation and extraction of crude fucoidan.
Sample preparation and extraction followed procedures described by Khoza et al. [14].
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