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The resulting DNA products are suitable for standard Illumina library preparation (end repair and ligation) (Fig. 1B).
All subsequent procedures, including mRNA purification, cDNA preparation, end repair of cDNA, adaptor ligation, and cDNA amplification were performed according to the manufacturer's protocols accompanying the mRNA-Seq Sample Preparation Kit (Illumina).
All procedures, including mRNA purification, cDNA preparation, end repair of cDNA, adaptor ligation, and cDNA amplification, were carried out according to the manufacturer protocols accompanying the mRNA-Seq Sample Preparation Kit (Cat. RS-930-1001, Inc.mina Inc., San Diego, CA, USA).
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But for those students, and tens of thousands of others who fell asleep on Wednesday with their No. 2 pencils at the ready, weeks of preparation ended with a fizzle.
I remember looking at the piles of bottled water in my garage after Hurricane Florence, and just for a moment, I thought, "Did we overprepare?" Several days of warning and preparation ended in a non-event for us, but we were lucky.
Eight hours of preparation ended in a quick flare-out after the grenades failed to penetrate the half-inch to three-quarter-inch-thick steel fuel tanks, Hoffmeister said.
Figure 3 shows the various steps of the FIB preparation, ending with the micropillar attached to the tip of a tomography pin.
The leisurely pace of these preparations ends resoundingly with the "Ballad of Mack the Knife," a stunning opening that signals that Weill's provocative musical soundscape, in all its dark dissonance and uneasy cheer, is in good hands.
Duration of switched use was calculated as the sum of use before switch and current use on the new preparation, until end date of defined daily doses of the last filled prescription or date of a study event.
"I don't see any difference in our preparation," defensive end Justin Tuck said, smiling.
Participants in the active discussion intervention showed improvements in functional status, anxiety, depression, and preparation for end of life.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com