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While kits have made extracting DNA and RNA from complex samples feasible in a laboratory setting, the manual steps and additional equipment requirements which often include an incubator, microcentrifuge, and physical lysis equipment make preparation difficult for personnel with limited training, few laboratory resources, or in remote locations.
This is especially true for breast tissues which contain a high content of fat, which makes sample preparation difficult.
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"Tropical storm conditions are expected to first reach the Florida west coast within the warning area later today, making outside preparations difficult or dangerous.
The South Koreans complained of not being issued visas until the last minute making preparations difficult, not being allowed to train on the same standard of fields as the host and staying in a noisy hotel.
People in threatened areas should start taking precautions immediately, as severe winds may hit Friday and make storm preparations difficult, the center urged. .
People in threatened areas should start taking precautions immediately, as severe winds may hit Friday and make storm preparations difficult, the center urged.
As deviations from the normal service process may be hard to anticipate, the resources and their preparation are difficult to manage in advance.
How much time should be devoted to preparation is difficult to determine.
In addition, although some of these catalysts have high TOF (e.g., solid silica based sulfuric acid [5] or SnIV TNH2PP)(OTf)2@CMP [11]), its preparation is difficult when compared with SANM.
However, the long months of preparation were difficult for Mackintosh.
Coach John Stevens is as studious as it gets but he said preparation is difficult to gauge.
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