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the preparation described in subparagraphs (A), (B), and (C) of subsection (d)(2).
The enzymatic preparation described here is superior to the existing chemical resolution route, exhibiting lower costs as well as higher yields, enantioselectivity, and substrate loads.
The electrochemical preparation described herein involved the electrocatalytic oxidation of sulfite on a platinum electrode modified with nanostructured copper salen (salen = N,N′-ethylenebis(salicylideneiminato)) polymer films.
mRNA was used for either 3′ RNA-seq library preparation (described below) or it was converted into cDNA to perform RT-qPCR analysis as follows69: Briefly, RNA retro-transcription (RT) was performed using SuperScript II (Life Technologies) and qPCR assays were performed using Platinum Quantitative PCR SuperMix-UDG or SYBR Green PCR Master Mix Life TechnologiesSYBR Green PCR Master Mix Life Technologies
The preparation of the urine and bile samples were the same as the plasma sample preparation described above.
The preparation, described today in the Proceedings of the National Academy of Sciences, has only been tested so far in animals.
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The lavish preparations described in Ms. Lombardi's article are, to say the least, very disheartening.
Cadmium oxide (CdO, Aldrich, 99.5%), selenium powder (Se, Aldrich, 95%), oleylamine (OA, Aldrich, 70%) were used in the preparations described here.
Together with the CGRP release preparations described previously, this allows to investigate the effect of chemical stimuli and antagonists on trigeminal afferents at all possible sites of action.
Alternatively, the entire bird (or any soft parts associated with preparations described above) may be preserved in alcohol.
Cells were isolated by micropipetting for the preparations described below.
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