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Its portfolio of products includes technologies for Polymerase Chain Reaction (PCR), sample preparation, cell culture, ribonucleic acid (RNA) interference analysis, functional genomics research, proteomics and cell biology applications, as well as clinical diagnostic applications, forensics and animal, food, pharmaceutical and water testing analysis.
Vector preparation, cell transfection, selection and purification procedures were performed essentially as described previously [29].
JFH-1 viral stock preparation, cell infection and titration was performed as described [12].
CDB participated to plasmids preparation, cell culture and Western blotting experiments.
PL carried out cell culture, sample preparation, cell apoptosis analysis and RT-PCR validation.
Work steps for PBMC preparation (cell isolation, freezing, thawing) were performed using laboratory SOPs.
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For inoculum preparation, cells were suspended in an isotonic solution and counted by a differential direct-count method in order to calculate the volume required [14].
For cytospin preparation cells were harvested by trypsinization.
For protein preparation, cells were washed by PBS, scrapped and collected.
For membrane preparation, cells were grown in multiple 15 cm culture dishes.
For LPS preparation, cells were grown in Erlenmeyer flasks at 37°C for 24 hrs in LB-broth with shaking.
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