Sentence examples for preparation by adding from inspiring English sources

We also controlled the purity of the preparation by adding an excess of ATP to extracts, which prevented virtually all of the proteins otherwise recovered in the ATP-fraction from binding to microtubules (Figure S1).

Intrasynaptosomal FURA PE 3-AM was determined for each synaptosomal preparation by adding 40 mM Mn2+ to quench the extracellular fluorescence; this Mn2+-quenched fluorescence comprised 7 10% of the total fluorescence at two wavelengths and was stable for the duration of the experiments.

Because the BoNT LC is a Zn-containing and Zn-dependent endopeptidase, enhancement of its catalytic activity was observed in some preparations by adding exogenous zinc (0.25 mM ZnCl2) depending on its zinc content.

In order to assess the impact of batch-to-batch variation on RNA circuit characterization, we tested three different extract/buffer preparations by adding a range of concentrations of the no-antisense control plasmid to 0.5 nM of the L1 plasmid.

After weaning, the mice were separated and put on different diets; 25 mice were fed a standard pellet diet and 20 mice/group were fed a pellet diet containing: MAK-4 (4%, w/w) MAK-5 (1%, w/w) and a combination of MAK-4+5 at the same concentrations (diets were prepared by adding commercial preparations of MAK-4 and MAK-5 to the pellet mixture, Piccioni, Milan).

Fatty acid methyl ester (FAME) preparation: A reagent solution was prepared by adding 20 µl of a 2M TMS diazomethane solution (Aldrich) to 1 ml of analytical grade dichloromethane (B&J).

FAM-loaded nanoparticles (FAM-Myr-NQA-MINs, FAM-NQA-MINs and FAM-NINs) were prepared by adding FAM during their preparation.

As reference, PPTF membranes were also prepared by adding CaCl2 to liquid PPP preparations using a molding glass chamber.

The LLO preparation was diluted by adding 10 μl of its preparation to a final volume of 1 ml of 2% SRBC suspension in PBS.

The uniform pore size of the catalysts was enlarged by varying the preparation conditions and by adding organic compounds such as N,N-dimethylformamide and formamide.

The preparation was performed by adding a Na-cit aqueous solution to an HAuCl4 aqueous solution at a constant temperature of 80°C under vigorous stirring.