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For the study of microvascular thrombus formation in vivo, we used the open cremaster muscle preparation, as previously described by our group [10, 11].
Total RNA from each sample and the universal reference RNA (Universal Mouse Reference RNA, Stratagene, http://www.stratagene.com) were amplified and used in probe preparation as previously described [9].
Total RNA from each sample (mouse or human) and the universal reference RNA Universal Humann or Mouse Reference RNA, Stratagene, http://www.stratagene.com) were amplified and used in probe preparation as previously described [27].
For Xyl, the 16-component mixture yielded more from AFEX-corn stover than either commercial preparation, as previously reported [ 5].
A modified Bligh and Dyer procedure was used to extract lipids from each mitochondrial preparation as previously described (Cheng et al., 2006).
Adult mice housed under standard conditions were anaesthetized 19 21 days after retroviral injection and processed for slice preparation as previously described (Ge et al., 2006).
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Sample preparation was as previously described.
A crude cell membrane preparation was prepared as previously described [ 23].
All cells were maintained at 37°C and 5% CO2 Nuclear preparations were prepared as previously described [ 25].
FISH analysis was carried out on metaphase chromosome preparations as previously described [16].
In h-S100A7 or LacZ-infected cells, levels of CTF-γ cleavage product of APP were assessed from membrane preparations, as previously described [20] [23].
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