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For example measurement of excursion, elongation and displacement of the brachial plexus nerves after reversed prosthesis surgery of the shoulder joint following model preparation as outlined in the present paper has previously been described in detail [12].
However, the long-range singleplex PCR amplification and NGS library preparation as outlined in Figures 1B (1) and B (2) are extremely labor intensive and time consuming.
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Call, write, or visit as outlined above.
Paired-end cDNA libraries for each RNA sample were prepared and sequenced using the Illumina TruSeq RNA Sample Preparation Kit, as outlined previously [ 11, 12].
Perhaps alternative approaches to vitespen preparation (such as outlined by Randazzo et al).
Synthesis of cDNA and library preparation was performed as outlined in the Illumina mRNA sequencing sample preparation guide (rev.
FDG PET/CT imaging and patient preparation was performed as outlined by Shankar et al. [22].
Both the DNA capture and library preparation was carried out as outlined in the protocols supplied by Agilent (protocol version 1.1.1) and Roche/nimblegen (NimbleGen SeqCap User's Guide, version 1.0, Illumina optimized), except that a Bioruptor (Diagenode) was used for fragmentation of the DNA.
Further growth, phage preparation, and panning were repeated as outlined above.
Afterwards, sample preparation and analysis were performed as outlined above.
For smaller tissue samples (less than 200 mg), the initial preparation and pulverization should be performed as outlined above.
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