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Product activities (A P) were measured after the end of preparation (approximately 15 min after the start of syntheses) and decay corrected to a typical injection time, 30 min after the start of synthesis (A P,30).
For each phage sample preparation, approximately 4×1011 phage particles were heated for 10 min in the SDS sample buffer (2% SDS, 10% glycerol, 0.67 M Tris-HCl, pH 6.8).
This resulted in a whole mount preparation approximately 6.5 cm in diameter.
Fifteen patients were within the window of bowel preparation (approximately two days before the surgery date) for surgery.
With the protocol used for antigen preparation approximately 108 E. cuniculi spores per ml gave a protein concentration in the final antigen solution of 0.3 mg/ml.
For each preparation, approximately 0.1 g of each sampled tissue, in triplicate, was chopped in Otto buffer I using straight-edge razor blades [ 23].
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Slow infusions using half-isotonic or isotonic preparations (approximately 1%) or small intermittent boluses of more concentrated preparations (approximately 8.4%) were preferentially used in later studies [13 15, 17, 18, 20] to avoid too rapid pH or osmolality changes, with no evidence of risk or benefit with either methods.
Instant thin layer chromatography was performed on all DTPA preparations approximately 30 min after reconstitution of the kit and at the time of dose administration.
Two different preparations of Bs20x22 (prepared approximately 4 months apart) were tested.
Briefly, 0.1 M Tris-HCl (pH 8.0), 0.3mM acetyl-Coenzyme A, 0.1mM DTNB, and samples of the mitochondrial preparation containing approximately 5 μg protein or whole-worm extract containing approximately 40 μg protein were incubated for 10 min at 24°C.
The entire bilateral standard procedure, including preparation, takes approximately 5 10 min.
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