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MACS, RNA preparation and transcript expression analysis were performed in PBTL as described ([14], and YL and NES, submitted).
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RNA extractions from staged larvae, cDNA preparations and transcript analysis by Q-PCR were performed with the TaqMan gene expression assay (Applied Biosystems) as described in Caldwell et al. (2005).
For in situ hybridization experiments, tissue preparation, sectioning and transcript detection were performed as described by Lozano et al. [79].
One potentially negative impact of ITR on the researcher is the additional time and effort required for transcript preparation and communication with interviewees.
Whereas efficiency and biases introduced by rRNA removal methods have been relatively well explored, the impact of cDNA synthesis and library preparation on transcript abundance remains poorly characterized.
The home page of ASGARD provides a brief description of the provenance and preparation of the transcript sequences house in the database.
Zebrafish embryos at different developmental stages were collected for cDNA preparation and subjected to RT-PCR using transcript specific primers (Fig. 3L).
In order to identify WAT depot-enriched transcripts, we undertook preparation and screening of murine suppressive subtractive hybridization (SSH) cDNA libraries enriched for genes expressed in either SC or EP murine adipocytes.
The standard RNA-seq library preparation protocols target poly-adenylated RNAs, thus restricting detection of SNPs to sequences encoding open-reading frames (ORFs) and transcript untranslated regions (UTRs).
In this preparation, the transcript for KGF expression was knocked down to 22%% of that by cells treated with non-targeted siRNA (Fig. 9a).
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