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Protein preparation and refinement was performed using Maestro 9.0 or 9.1 Protein Preparation Wizard, and default parameters were used to optimize protein structures.
EHs are also used in the preparation and refinement of enantiopure diols and epoxides, which are important synthons in organic synthesis.
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The 3D structure was processed by use of the 'Protein Preparation module' with the 'preparation and refinement' option before docking.
BM, DL contributed to documentation and refinement of study methods and manuscript preparation.
The modelled structure was imported and corrections were carried out by Protein Preparation wizard of Schrodinger, where hydrogens were added automatically and refinement of the structure was done.
79 Blood injections have been recognised as potentially therapeutic in sports medicine for over 60 years, 80 and refinement of this in the form of autologous plasma preparations (platelet-rich plasma) has recently been popularised for muscle strain injuries.
Interest in incorporating rare earth ions into KPb2Cl5 has lead to further refinements of material preparation and crystal growth techniques [28 31].
In recent years, refinements in autoantigen preparation and better selection of polyclonal and monoclonal antibodies have led to a new (third) generation of automated quantitative immunoassays (IMAs) with improved sensitivity and specificity for the measurement of TPOAbs [4, 5].
The combination of automated sample preparation and real-time PCR offers such refinements.
Therefore, although sites were technically working in the Implementation stage, by design, rapid-cycle testing allowed recursive movement such that sites could cycle to the earlier Exploration and Preparation stages as refinements to agency plans and procedures were made.
Its lavish appointments suggest breeding and refinement.
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