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It required less time for sample preparation and quantification compared to HPLC.
After 454 library preparation and quantification of the libraries by qPCR using emPCR priming sites [44], sequencing was carried out on the 454 FLX platform.
RNA-Skim includes two stages, preparation and quantification.
Preparation and quantification of RNA and cDNA were by MLK, DJC, and REO.
Preparation and quantification of DNA were by EJM, CMF, AWG, LAH, and AMW.
The workflow of RNA-Skim includes two stages: preparation and quantification.
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Quality control of the RNA preparations and quantification of total RNA amount was done using an Agilent 2100 bioanalyzer (Agilent Technologies, USA, Santa Clara) and a NanoDrop 1000 spectrophotometer.
The muscle dissection, preparation, staining, and quantification of the neuromuscular synapses have been previously described [29], [31].
preparation, visualisation and quantification of mitotic and s-phase indices were as described previously [34] Proteins from SDS PAGE were blotted onto Hybond ECL (Amersham) and developed with Supersignal West Pico (Pierce).
Here we show for the first time that ATR-FT-IR spectroscopy, in combination with partial least-squares regression models, has the required ease of sample preparation, sensitivity, and quantification ability to become a laboratory standard for malaria detection and most importantly quantification.
The pITR plasmid was constructed, purified and used to prepare dilutions for standard curve preparation and DNA quantification.
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