Three independent culture preparations were prepared and pooled to address biological variation.
Sample were pooled from three independent cell preparations (for NHE1 shRNA, two different shRNA constructs were tested in two cell preparations and pooled).
Two groups of total RNA were used for library preparation and sequencing by pooling equal quantity (10 μg) of total RNA isolated from six individual multiple and uniparous goats ovaries.
Two groups of total RNA were used for library preparation and sequencing by pooling equal quantity (10 μg) of total RNA isolated from six individual pregnant or non-pregnant goat ovaries.
"We found resistant bacteria in public water used for drinking, washing and food preparation and also in pools and rivulets in heavily-populated areas where children play," he said.
To assess the efficacy of the SI model of training faculty from this broader diversity of institutions, which differ in mission, student preparation, and resources, we pooled our STAR survey respondents into two groups based on the Carnegie undergraduate instructional program categories.
For targeted enrichment by hybrid capture, indexing of the sample is usually performed after the enrichment but to reduce the number of enrichment reactions, the sample libraries can alternatively be indexed during the library preparations and then pooled for enrichment [ 15].
Seventy-five 3-L bacterial culture preparations were made and pooled for the AT-1 batch (568 mL) and twenty-four 3-L baculture culture preparations were made and pooled for the AT-2 batch (169 mL).
This would be extremely laborious in DNA pool preparation and PCR screening.
