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See Table 1 for a summary of the key patient preparation and image acquisition parameters.
The flow sensitive preparation and image orientation are also not connected as they are in conventional time-of-flight techniques.
Both tissue section preparation and image analysis can be automated in the future (Périn C., Henry A. pers com).
Nevertheless, studies with long time series or large sample sizes are not very common for diffuse-porous tree species due to the laborious sample preparation and image analysis.
At the National Center for X-ray Tomography (NCXT), specimen preparation and image collection for tomographic reconstruction of a whole cell require only minutes.
Also, mass spectrometry imaging (MSI) increased its application in several science areas, since improvements in instrumentation, sample preparation and image software has been carried out [5].
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Whole mounts of tail epidermis, tissue preparation, and images were acquired as described previously (Jensen et al., 2009).
The revised manuscript now contains more experimental details, a new figure that depicts the process of sample preparation and images to give more insight into the geometry of the micro compartments.
This article reviews the requirements in terms of specimen preparation, imaging, and image analysis for quantitative FSM (qFSM) experiments.
While issues related to specimen preparation, imaging, and image interpretation make this approach to cell architecture difficult, recent improvements in methods, equipment, and software have facilitated the study of both important macromolecular complexes and comparatively large volumes from cellular specimens.
Evaluation of the immunohistochemistry preparations and image acquisition was performed using an Olympus BX50 light microscope.
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