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Endogenous control genes are used to estimate variations and experimental errors occurring during sample preparation and expression measurements.
Sample preparation and expression analysis has been described elsewhere (Butt et al., 2014; Korbei et al., 2013).
Samples, cDNA preparation and expression analysis Samples of total RNA from human tissues were purchased from Clontech (Mountain View, CA, USA) and BioChain Institute (Hayward, CA, USA).
The experimental design of biological sample preparation and expression profile analysis of temperature and acidification was carried out by PJA, AT and VL.
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Potential targets of such protective immunity have been identified by screening crude antigen preparations and expression libraries with sera from animals vaccinated with irradiated L3s [ 11, 12].
MACS, RNA preparation and transcript expression analysis were performed in PBTL as described ([14], and YL and NES, submitted).
ABZ contributed to the RNA preparation and the expression analyses.
The plant material preparation and gene expression analyses were carried out by DB, RS and SH.
The plant material preparation and gene expression analyses were carried out by RL.
Additional information 2. Details of sample preparation and gene expression profiling.
Data analysis includes peak recognition, calibration curve preparation, and protein expression level and limit of quantification calculations.
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