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Additional file 1: Summary statistics for Illumina HiSeq, read preparation and alignment to the honey bee genome.
Shear bond strength evaluation requires easier specimen preparation and alignment during measurement than microtensile bond strength measurement [ 29].
Subclonal variants can be detected using the deep coverage of next-generation sequencing technologies, but their distinction from sequencing errors, library preparation and alignment artifacts suffers from an unfavorable signal to noise level (Gerstung et al., 2012; Schmitt et al., 2012).
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Further details on library preparation, sequencing and alignment can be retrieved from Kranis et al. [ 34].
Due to various error sources and technical limitations of library preparation, sequencing, and alignment, however, a substantial level of noise complicates the analysis.
There is minimal loss of sensitivity or specificity with 2 × 75 cycle sequences when compared with 2 × 101 cycles using current library preparation methods and alignment and variant calling algorithms.
Haplotype-resolved genome sequencing was performed for each parent using a modified Long Fragment Read protocol [ 11] involving limiting dilution of DNA into 96-well plates followed by amplification, library preparation, sequencing, and alignment (Additional file 1: Note S1).
The mismatch rate δ i = β i + ε i may be decomposed into biological and technical variation, where ε i denotes the technical error that accumulated during the preparation, sequencing and alignment steps and β i denotes the biological nucleotide variation at site i.
The lack of mitochondrial genomes produced by most high-throughput sequencing projects is most likely based on biases against the AT-rich and repetitive DNA during library preparation, sequencing and alignment [ 34, 35] and discussed in [ 27], but complete mtDNA sequence reconstruction is possible [ 32].
Compared to conventional cutting guides, semi-autonomous robotic technologies have improved the precision of bone preparation and component alignment in UKA, enabling successful early functional results and with the expectation that they will enhance implant durability.
Preparation, transfer, cooling and alignment of the photocathode are discussed.
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