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This original substrate preparation allowed to perform electrochemical measurements on ionized species in solution (in this case, potassium chloride) with decreasing concentration down to 10−7 molar.
This original substrate preparation allowed to perform electrochemical measurements on ionized species in solution with decreasing concentration down to 10−7 molar.
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All the preparations for labeling and MS analysis were shown to contain adequate amounts of glycans and their labeled ones, respectively (data not shown) indicating that less than 1 μL of the labeled preparations allowed to analyze most of the N-glycans expressed on the AGP molecule irrespective of their preparations both from healthy controls and patients with cancer as described below.
After preparation of the exteriorized cremaster muscle, the tissue was superfused with low oxygen Krebs' solution and the preparation was allowed to rest for the next 15-20 minutos tobtainin steady state.
While investigations were under way, all work had to stop and no food preparation was allowed to restart until yesterday morning.
Combination of the preparation conditions allowed to obtain series of the carbon with textural characteristics varied in the wide range.
Variation of the preparation conditions allowed to obtain carbon materials with surface area varied in wide range, from 540 to 3060 m2/g and, which should be emphasized especially, with close values of the micropore fraction (20 30 %).
A preload tension of 2 g was applied to each preparation and allowed to equilibrate for 1 h.
The resting tension was fixed at 1 g and the preparation was allowed to stabilization for 30 min.
The preparation was allowed to equilibrate for about 1 h to reach a stable beating rate before responses to CCh or Iso were determined.
A preload tension of 2 g was applied to each preparation and allowed to equilibrate for a period of 1 hour.
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