The films were first characterized immediately after their preparation and afterwards were allowed to oxidize in air at room temperature for a given time to study their aging.
"Every interview stage involved so much research and mental preparation, exhaustion afterwards, waiting, waiting and more waiting, plus … psyching yourself up for each next stage," Martins said of the three-month process.
Afterwards, preparation of the glenoids was continued.
Afterwards, preparations began for the battalion's return to New Zealand.
Afterwards preparations were postfixed with 2% osmium tetraoxide in 0.1 M cacodylate buffer for 2 hours at 4°C.
The retrieved implants were cleaned with sterile water and afterwards prepared according to the same preparation protocol applied to the other inlays.
Afterwards, sample preparation and analysis were performed as outlined above.
Afterwards, the preparation chamber was cooled down to room temperature in the case of the lipid mixture and carefully rinsed with 10 mM NaH2PO4 pH 7.6, to remove the water-soluble ATTO 647 that was not enclosed in the interior of the vesicles.
Afterwards, the preparations were freeze-dried, and working solutions with concentrations of extract equal to 1 mg/ml were prepared.
"They gave me time off for the [track] world championships and then to come back for the classics afterwards without optimal preparation for, say, Milan-San Remo.
Objectives: To assess the diagnostic reality for rare hantavirus infections in Europe by (1) running a pre-evaluation panel (four samples, sent out in 1999) to optimise sample preparation and shipping procedure and afterwards (2) starting an External Quality Assurance (EQA) program (20 samples, sent out in 2001).
